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Biochemistry

Biomedical research on foods, nutrition, and lifestyle-related diseases

Prevention of cancer and other lifestyle-related diseases by food components and nutritional intervention

Lifestyle-related diseases such as cancer, diabetes, and cardiovascular diseases are increasing worldwide. Oxidative stress and hyperlipidemia are features common to these diseases. Reactive oxygen and nitrogen species and lipid peroxidation products generated in inflamed tissues can react with biomolecules such as DNA and proteins to form adducts, leading to their functional impairments. We use biochemical and molecular biology methods (e.g., LC-MS/MS, proteomics, and genomics techniques) to identify novel biomarkers (e.g., adducts, abnormal proteins) which are associated with early onset lifestyle-related diseases. Using these biomarkers, we also search for food and nutritional factors that prevent and delay the onset of lifestyle- and aging-related diseases.


1. Cancer chemoprevention by food constituents and nutrients and their underlying molecular mechanisms
We investigate the chemopreventive effects of food constituents and nutrients, especially those present in Shizuoka-local foods such as Japanese yam, using animal models of carcinogenesis and biochemical and molecular biology techniques.
2. Evaluation of health foods and dietary supplements using biochemical parameters
We aim to establish a system for assessing the effects of dietary supplements on human health using various biochemical parameters.
3. Biomarkers for developing cancer and other lifestyle related diseases
We identify the structural and functional modifications of biomolecules (DNA and proteins) which are formed by reactions with reactive oxygen species generated in inflamed tissues as well as with environmental and endogenous carcinogens. These modified biomolecules (e.g., adducts) could be measured to study the etiology, diagnosis, and prevention of lifestyle-related diseases.
4. Molecular mechanisms and prevention of lifestyle related diseases
We investigate the pathogenic mechanisms for diabetes and other lifestyle-related diseases using biochemical and molecular biology techniques in order to establish new preventive strategies. In particular we search for chemopreventive agents in Shizuoka-local foods and examine their effectiveness using animal models and in human clinical studies.

Professor

Noriyuki MiyoshiPhD, Assoc. Prof.
miyoshin@u-shizuoka-ken.ac.jp
TEL.+81-54-264-5531

Professor

Tsutomu HashizumePhD

Details are here

http://dfns.u-shizuoka-ken.ac.jp/labs/biochem/index.html

 

Figure.1

Prevention of azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice with diosgenin, a major phytosterol present in yam. (Cancer Prev. Res., 2011).

Figure.2

Formation of cholesterol ozonolysis products, secosterol-A and –B, measured by LC-MS/MS as biomarkers of inflammation, in plasma samples of mice after i.p. injection of lipopolysaccharide (J. Lipid Res. 2011).

Publications

  1. Biosci. Biotechnol. Biochem., (2016) in press
  2. J. Chromatogr. B, (2015) 988, 149-56.
  3. Biochem. Biophys. Res. Commun., (2014) 446, 702-8.
  4. Free Radic. Biol. Med., (2013) 60, 73-9.
  5. Biosci. Biotechnol. Biochem., (2013) 77, 651-3.
  6. Anal. Biochem. (2012) 429, 124-31.
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