食品化学研究室 −産業応用を見据えた食品機能開発化学の推進

 

原著論文(2010年以降)
H. Mizuno, Y. Miyazaki, K. Ito, K. Todoroki, J.Z. Min, T. Toyo'oka. A rapid and sensitive detection of D-Aspartic acid in Crystallin by chiral derivatized liquid chromatography mass spectrometry. J. Chromatogr. A 2016 in press
Y. Kuroda, R. Ikeda, T. Yamazaki, K. Ito, K. Uda, K. Wakabayashi, T. Watanabe. Activation of human bitter taste receptors by polymethoxylated flavonoids. Biosci. Biotechnol. Biochem. 2016 4 1-4.
M. Ohno, K. Ito, V.T.T. Lan, M. Kusubata, C. Tometsuka, Y. Koyama, T. Motoyama, S. Ito, Y. Kawarasaki. Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides. Peptides 2015 69 115-117.
V.T.T. Lan, K. Ito, M. Ohno, T. Motoyama, S. Ito, Y. Kawarasaki. Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor. Food Chem. 2015 15(175) 66-73.
V.T.T. Lan, K. Ito, S. Ito, Y. Kawarasaki. Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV. Peptides 2014 54 166-170.
T. Kurose, Y. Saito, K. Kimata, Y. Nakagawa, A. Yano, K. Ito, Y. Kawarasaki. Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: a sub-milligram production of "difficult-to-express" secretory protein. J. Biosci. Bioeng. 2014 117(6) 659-663.
K. Ito, A. Hikida, V.T.T. Lan, T. Motoyama, S. Kitagawa, Y. Yoshikawa, R. Kato, Y. Kawarasaki. Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library. Nature Commun. 2013 4(2502).
M. Hoshi, Y. Ohki, K. Ito, T. Tomita, T. Iwatsubo, Y. Ishimaru, K. Abe, T. Asakura. Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana. BMC Biochemistry 2013 14(16).
A. Hikida, K. Ito, T. Motoyama, R. Kato, Y. Kawarasaki. Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system. Biochem. Biophys. Res. Commun. 2013 430(4) 1217-1222.
K. Ito, A. Hikida, S. Kitagawa, T. Misaka, K. Abe, Y. Kawarasaki. Soy peptides enhance heterologous membrane protein expression during the exponential growth phase of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 2012 76(3) 628-631.
K. Kimata, M. Yamaguchi, Y. Saito, H. Hata, K. Miyake, T. Yamane, Y. Nakagawa, A. Yano, K. Ito, Y. Kawarasaki. High cell-density expression system: A novel method for extracellular production of "difficult-to-express" proteins. J. Biosci. Bioeng. 2012 113(2) 154-159.
A. Koizumi, A. Tsuchiya, K. Nakajima, K. Ito, A. Shimizu-Ibuka, T. Asakura, L. Briand, T. Misaka, K. Abe. Human sweet taste receptor mediates acid-induced sweetness of miraculin. Proc. Natl. Acad. Sci. USA 2011 108(40) 16819-16824.
K. Nakajima, A. Koizumi, K. Iizuka, K. Ito, Y. Morita, T. Koizumi, T. Asakura, A. Shimizu-Ibuka, T. Misaka, K. Abe. Non-acidic compounds induce the intense sweet taste of neoculin, a taste-modifying protein. Biosci. Biotechnol. Biochem. 2011 75(8) 1600-1602.
H. Asada, T. Uemura, T. Yurugi-Kobayashi, M. Shiroishi, T. Shimamura, H. Tsujimoto, K. Ito, T. Sugawara, T. Nakane, N. Nomura, T. Murata, T. Haga, S. Iwata, T. Kobayashi. Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis. Microb. Cell Fact. 2011 22;10:24.
K. Nakajima, K. Yokoyama, T. Koizumi, A. Koizumi, T. Asakura, T. Terada, K. Masuda, K. Ito, A. Shimizu-Ibuka, T. Misaka, K. Abe. Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein. PLoS ONE 2011 6(4) e19448.
N. Tokuda, K. Igarashi, T. Shimamura, T. Yurugi-Kobayashi, M. Shiroishi, K. Ito, T. Sugawara, H. Asada, T. Murata, N. Nomura, S. Iwata, T. Kobayashi. Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium. Protein Expr. Purif. 2011 79(1) 81-87.
T. Asakura, M. Miyano, H. Yamashita, T. Sakurai, K. Nakajima, K. Ito, T. Misaka, Y. Ishimaru, K. Abe. Analysis of taste continuity - case of sweet proteins. Flav. Fragr. J. 2011 26(4) 274-278.
K. Ito, S. Ito, T. Shimamura, S. Weyand, Y. Kawarasaki, T. Misaka, K. Abe, T. Kobayashi, A.D. Cameron, S. Iwata. Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans. J. Mol. Biol. 2011 408(2) 177-186.
K. Ito, T. Sugawara, A. Koizumi, K. Nakajima, A. Shimizu-Ibuka, M. Shiroishi, H. Asada, T. Yurugi-Kobayashi, T. Shimamura, T. Asakura, K. Masuda, M. Ishiguro, T. Misaka, S. Iwata, T. Kobayashi, K. Abe. Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin. Biochim. Biophys. Acta - General Subjects 2010 1800(9) 986-992.
T. Sakurai, T. Misaka, M. Ishiguro, K. Masuda, T. Sugawara, K. Ito, T. Kobayashi, S. Matsuo, Y. Ishimaru, T. Asakura, K. Abe. Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16. J. Biol. Chem. 2010 285(36) 28373-28378.
K. Ito, S. Ito, T. Shimamura, Y. Kawarasaki, K. Abe, T. Misaka, T. Kobayashi, S. Iwata. Crystallization and preliminary X-ray analysis of a Glucansucrase from the dental caries pathogen Streptococcus mutans. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2010 66(Pt 9) 1086-1088.
K. Ito, T. Sugawara, A. Koizumi, K. Nakajima, A. Shimizu-Ibuka, M. Shiroishi, H. Asada, T. Yurugi-Kobayashi, T. Shimamura, T. Asakura, T. Misaka, S. Iwata, T. Kobayashi, K. Abe. Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein. Biotechnol. Lett. 2010 33(1) 103-107.
M. Miyano, H. Yamashita, T. Sakurai, K. Nakajima, K. Ito, T. Misaka, Y. Ishimaru, K. Abe, T. Asakura. Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model. J. Agric. Food Chem. 2010 58(22) 11870-11875.