Original papers and reviews published in
international journals
*Kawarasaki Y., Ohashi S., Watabe R., Tanaka M., Kurose T., Ito K.: High
Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently
Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant
Proteins. In: Garcia Fruitós E., Arís Giralt A. (eds) Insoluble
Proteins. Methods in Molecular Biology, vol 2406. Humana, New York, NY.
(2022) doi: 10.1007/978-1-0716-1859-2_16.
Tanaka M., Ito K., Matsuura T., Kawarasaki Y., Gomi K. Identification
and distinct regulation of three di/tripeptide transporters in
Aspergillus oryzae. Biosci. Biochem. Biotechnol., 85 (2), 452-463 (2021)
Ito K., Hosoya T., Yamazaki-Ito T., Terada Y., Kawarasaki
Y. Dipeptidyl peptidase IV inhibitory dipeptides contained in
hydrolysates of green tea grounds. Food Science and Technology Research
27 (2), 329-334 (2021)
Ito K. Koike M., Kuroda Y., Yamazaki-Ito T., Terada Y., Ishii T.,
Nakamura Y., Watanabe T., Kawarasaki Y.: Bitterness-masking peptides for
epigallocatechin gallate identified through peptide array analysis.
Food Science and Technology Research 27 (2), 221-228
(2021)
Su H, Ito K, Kawarasaki Y, Morita H, Nose H, Ikeda K, Nakadouzono F,
Gokuden M, Kamiyama S, Tokaji A, Rikitake Y, Kawaguchi T, Umekita K,
Oishi S, Abe F, Kanda T, Kawabata H, Ando S, Ohashi N. Insight of
diagnostic performance using B-cell epitope antigens derived from triple
P44-related proteins of Anaplasma phagocytophilum. Diagn. Microbiol.
Infect. Dis. 2019 Oct;95(2):125-130 (2019). doi:
10.1016/j.diagmicrobio.2019.05.008. Epub 2019
Hashidume T, Sakano T, Mochizuki A, Ito K, Ito S, Kawarasaki Y, *Miyoshi
N.: Identification of soybean peptide leginsulin variants in different
cultivars and their insulin-like activities. Sci Rep. 2018 Nov
15;8(1):16847. doi: 10.1038/s41598-018-35331-5.
Ohno M., Ito K., Lan V.T.T., Kusubata M., Tometsuka C., Koyama Y.I.,
Motoyama T., Ito S., Kawarasaki Y.: Synergistic inhibition of human
dipeptidyl peptidase IV by combinations of peptides. Peptides 69,
115-117, (2015)
Mori A., Hara S., Sugahara T., Kojima T., Iwasaki Y., Kawarasaki Y.,
Sahara T., Ohgiya S., Nakano H.: Signal peptide optimization tool for
the secretion of recombinant protein from Saccharomyces cerevisiae. J
Biosci Bioeng. 120, 518-525 (2015)
Lan V.T.T., Ito K., Ohno M., Motoyama T., Ito S.,
Kawarasaki Y.: Analyzing a dipeptide library to identify
human dipeptidyl peptidase IV inhibitor. Food Chem. 175,
66-73 (2015)
Mori A., Hara S., Sugahara T., Kojima T., Iwasaki Y.,
Kawarasaki Y., Sahara T., Ohgiya S., Nakano H.: Signal
peptide optimization tool for the secretion of recombinant
protein from Saccharomyces cerevisiae. J Biosci Bioeng.
120, 518-525 (2015)
Ohno M., Ito K., Lan V.T.T, Kusubata M., Tometsuka C.,
Koyama Y.I., Motoyama T., Ito S., Kawarasaki Y.:
Synergistic inhibition of human dipeptidyl peptidase IV by
combinations of peptides. Peptides 69, 115-117, (2015)
Lan V.T.T., *Ito K., Ito S., and Kawarasaki Y.:
Trp-Arg-Xaa tripeptides act as uncompetitive-type
inhibitors of human dipeptidyl peptidase IV, Peptides, 54,
166-70 (2014)
*Kawarasaki Y., Kurose T., Ito K.: High cell-density
expression system: Yeast cells in a phalanx efficiently
produce a certain range of “difficult-to-express”
secretory recombinant proteins, Method. Mol. Biol.
“Insoluble Proteins: Methods and Protocols” Chapter 8, ed.
by Elena Garcia-Fruitos, Humana Press (2014)
Kurose T., Saito Y., Kimata K., Nakagawa Y., Yano A., Ito
K., and *Kawarasaki Y.: Secretory expression of Lentinula
edodes intracellular laccase by yeast high-cell-density
system: a sub-milligram production of
“difficult-to-express” secretory protein. J. Biosci.
Bioeng. (DOI: 10.1016/j.jbiosc.2013.11.014).
*Ito K., Hikida A., Kawai S., Lan V.T.T., Motoyama T.,
Kitagawa S., Yoshikawa Y., Kato R., Kawarasaki Y.:
Analysing the substrate multispecificity of a
proton-coupled oligopeptide transporter using a dipeptide
library. Nat. Commun. 4, 2502 (2013).
Hikida A., *Ito K., Motoyama T., Kato R., Kawarasaki Y.:
Systematic analysis of a dipeptide library for inhibitor
development using human dipeptidyl peptidase IV produced
by a Saccharomyces cerevisiae expression system. Biochem.
Biophys. Res. Commun. 430, 1217-22 (2013)
*Ito K., Hikida A., Kitagawa S., Misaka T., Abe K., and
Kawarasaki Y.: Soy peptides enhance heterologous membrane
protein expression during the exponential growth phase of
Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 76,
628-631 (2012)
Hoshi M., Ohki Y., Ito K., Tomita T., Iwatsubo T.,
Ishimaru Y., Abe K., Asakura T.: Experimental detection of
proteolytic activity in a signal peptide peptidase of
Arabidopsis thaliana. BMC Biochemistry 14, 16 (2013).
Kimata K., Yamaguchi M., Saito Y., Hata H., Miyake K.,
Yamane T., Nakagawa Y., Yano A., Ito K., and *Kawarasaki
Y., High cell-density expression system: A novel method
for extracellular production of "difficult-to-express"
proteins. J. Biosci. Bioeng.,113, 154-159 (2012)
Kojima T., Nagao N., Ando D., Ojima T., Kawarasaki Y.,
Kobayashi I., Nakajima M., *Nakano H. Emulsion culture: A
miniaturized library screening system based on
micro-droplets in an emulsified medium. J. Biosci.
Bioeng.112, 299-303 (2011)
Ito K., *Ito S., Shimamura T., Weyand S., Kawarasaki T.,
Misaka T.,
Abe K., Kobayashi T., Cameron A., and Iwata S. Crystal
structure of
glucansucrase from dental caries pathogen Streptococcus
mutans. J. Mol.
Biol., 408, 177-186 (2011)
Koizumi A., Tsuchiya A., Nakajima K., Ito
K., Shimizu-Ibuka A., Asakura T., Briand L., Misaka T.,
Abe K., Human sweet taste receptor mediates acid-induced
sweetness of miraculin. Proc. Natl. Acad. Sci. USA 108,
16819-16824 (2011)
Nakajima K., Koizumi A., Iizuka K., Ito K., Morita Y.,
Koizumi T., Asakura T., Shimizu-Ibuka A., Misaka T., Abe
K., Non-acidic compounds induce the intense sweet taste of
neoculin, a taste-modifying protein. Biosci. Biotechnol.
Biochem. 75, 1600-1602 (2011)
Asada H., Uemura T., Yurugi-Kobayashi T., Shiroishi M.,
Shimamura T., Tsujimoto H., Ito K., Sugawara T., Nakane
T., Nomura N., Murata T., Haga T., Iwata S., Kobayashi T.,
Evaluation of the Pichia pastoris expression system for
the production of GPCRs for structural analysis. Microb.
Cell Fact. 22, 10-24 (2011)
Nakajima K., Yokoyama K., Koizumi T., Koizumi A., Asakura
T., Terada T., Masuda K., Ito K., Shimizu-Ibuka A., Misaka
T., Abe K., Identification and modulation of the key amino
acid residue responsible for the pH sensitivity of
neoculin, a taste-modifying protein. PLoS ONE 6, e19448
(2011)
Tokuda N., Igarashi K., Shimamura T., Yurugi-Kobayashi T.,
Shiroishi M., Ito K., Sugawara T., Asada H., Murata T.,
Nomura N., Iwata S., Kobayashi T., Cloning, expression and
purification of the anion exchanger 1 homologue from the
basidiomycete Phanerochaete chrysosporium. Protein Expr.
Purif. 79, 81-87 (2011)
Asakura T., Miyano M., Yamashita H., Sakurai T., Nakajima
K., Ito K., Misaka T., Ishimaru Y., Abe K., Analysis of
taste continuity – case of sweet proteins. Flav. Fragr. J.
26, 274-278 (2011)
Kamiya T, Ojima T, Sugimoto K, Nakano H, *Kawarasaki Y.
Quantitative
Y2H screening: Cloning and signal peptide engineering of a
fungal
secretory LacA gene and its application to yeast
two-hybrid system as a
quantitative reporter. J. Biotechnol. 146，151-159, (2010)
Ikeuchi A., Kamiya T., Sugimoto K., Yamane T., Nakano H.,
and
*Kawarasaki Y., A method for reverse interactome analysis:
High-resolution mapping of interdomain interaction network
in Dam1
complex and its specific disorganization based on
interaction domain
expression. Biotech. Progr. 26, 945-53 (2010)
Ito K., Ito S., Shimamura T., Kawarasaki Y., Abe K.,
Misaka T.,
Kobayashi T., and Iwata S., Crystallization and
preliminary X-ray
analysis of a glucansucrase from the dental caries
pathogen
Streptococcus mutans. Acta Cryst. F66, 1086-1088 (2010)
Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P.,
Ribnicky B.,
Palmer T., and Georgiou G*., J. Biol. Chem., 282、8306-16
(2007)
Zhao M., Zhou L., Kawarasaki Y., and Georgiou G.*:
Regulation of RraA,
a protein of RNase E-mediated RNA decay., J. Bacteriol.,
188,
3257-3263, (2006)
Griswold K., Kawarasaki Y., Ghoneim N., Benkovic S.,
Iverson B.L., and
Georgiou G.*: Evolution of Highly Active Enzymes by
Homology-Independent Recombination, Proc. Natl. Acad. Sci.
USA, 102,
10082-10087 (2005)
Kojima T., Takei Y., Ohtsuka M., Kawarasaki Y., Yamane T.,
Nakano T.*:
PCR amplification from single DNA molecules on magnetic
beads in
emulsion: application for high-throughput screening of
transcription
factor targets., Nucl. Acid. Res. 33, e150 (2005)
Jeong K.J, Kawarasaki Y, Gam J, Harvey BR, Iverson BL,
Georgiou G.*:
Periplasmic fluorescent reporter protein and its
application in
high-throughput membrane protein topology analysis. J.
Mol. Biol., 341,
901-9 (2004)
Yasuaki Kawarasaki*, Yasuhiro Yamada, Maki Ichimori,
Tomoya Shinbata,
Katsunori Kohda, Hideo Nakano, Tsuneo Yamane:
Stabilization of affinity
tagged recombinant protein during/after its expression in
cell-free
protein synthesis system using wheat-germ extract., J.
Biosci. Bioeng.,
95, 209-214 (2003)
Akinori Ikeuchi, Yasuaki Kawarasaki*, Tomoya Shinbata, and
Tsuneo
Yamane: Chimeric Gene Library Construction by a Simple and
Highly
Versatile Method Using Recombination-Dependent Exponential
Amplification. Biotechnol. Progr., 19, 1460-1467 (2003)
Yasuaki Kawarasaki, Karl E. Griswold, James D. Stevenson,
Tzvia Selzer,
Stephen J. Benkovic, Brent Iverson, and George Georgiou*:
Enhanced
Crossover SCRATCHY: Construction and High-throughput
Screening of a
Combinatorial Library Containing Multiple Non-Homologous
Crossovers.
Nucl. Acid. Res., 31, e126, (2003)
Akinori Ikeuchi, Yoko Sasaki, Yasuaki Kawarasaki*, and
Tsuneo Yamane:
Exhaustive identification of interaction domains using a
high-throughput method based on two-hybrid screening and
PCR-convergence: Molecular dissection of a kinetochore
subunit
Spc34p. Nucl. Acid. Res., 31, 6953-6962, (2003)
Denfu Chen, Yasuaki Kawarasaki, Hideo Nakano, and Tsuneo
Yamane*:
Cloning and in vitro and in vivo expressions of plant
glutathione
S-transferase Zeta class genes. J. Biosci. Bioeng., 95,
594-600 (2003)
Yasuaki Kawarasaki*, Yoko Sasaki, Akinori Ikeuchi, Shoji
Yamamoto and
Tsuneo Yamane: A method for functional mapping of
protein-protein
binding domain by preferential amplification of the
shortest amplicon
using PCR, Analytical Biochemistry, 303, 34-41 (2002)
Suang Rungpragayphan, Yasuaki Kawarasaki, Takao Imaeda,
Katsunori
Kohda, Hideo Nakano and Tsuneo Yamane*: High-throughput,
Cloning-independent Protein Library Construction by
Combining
Single-molecule DNA Amplification with in Vitro
Expression, J. Mol.
Biol., 318, 395-405 (2002)
Yugo Iwasaki, Tatsuya Nishiyama, Yasuaki Kawarasaki, Hideo
Nakano, and
Tsuneo Yamane*: Importance of Disulfide Bridge Formation
on Folding of
Phospholipase D from Streptomyces antibioticus, Journal of
Bioscience
and Bioengineering, 89, 506-508 (2000)
Yasuaki Kawarasaki*, Satoru Kasahara, Natsuko Kodera,
Tomoya Shimbata,
Satoshi Sekiguchi, Hideo Nakano, and Tsuneo Yamane: A
trimmed viral
cap-independent translation enhancing sequence for a rapid
in vitro
gene expression, Biotechnology Progress., 16, 517-521
(2000)
河原崎泰昌、山田恭裕、市森真紀、新畑智也、幸田勝典、中野秀雄、山根恒夫 「Stabilization of
Affinity-Tagged
Recombinant Protein during/after its Production in a
Cell-free System
using Wheat-germ Extract
（小麦胚芽無細胞タンパク質合成系で生産されるタグ付き組換えタンパク質の安定化）」日本生物工学会 会誌、第８３巻３号、
P135 （２００５）
