研究業績

2017年

[原著論文]

Tomoharu Motoyama1, Shogo Nakano1*, Yuta Yamamoto, Hiroaki Tokiwa, Yasuhisa Asano, Sohei Ito, Product release mechanism associated with structural changes in monomeric L-threonine 3-dehydrogenase, Biochemistry, in press, DOI: 10.1021/acs.biochem.7b00832, 1Equally contribution, *Corresponding author

・Daisuke Matsui*, Shogo Nakano*, Mohammad Dadashipour, and Yasuhisa Asano, Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity, Scientific reports, 7, 9558, doi:10.1038/s41598-017-09749-2 *Equally contribution

[日本語総説・解説]

中野 祥吾、ウェット・ドライ融合によるタンパク質工学研究の今、生物工学会誌 バイオミディア(日本生物工学会)95, 22


2016年

[原著論文]

Shogo Nakano, Kazuyuki Yasukawa, Takaki Tokiwa, Takeshi Ishikawa, Erika Ishitsubo, Naoya Matsuo, Sohei Ito, Hiroaki Tokiwa, and Yasuhisa Asano, Origin of stereoselectivity and substrate/ligand recognition in an FAD-dependent R-selective amine oxidase, J. Phys. Chem. B., 120, 10736-10743, (2016)

[特許]

・ 浅野 泰久、中野 祥吾伊藤 創平本山 智晴松永 玲実、特願2016-234857

[学会発表]

中野 祥吾, Wet-Dry融合による新たな蛋白質工学的手法の開発 ~INTMSAlignの開発と応用~, 2016年度日本生物工学会 中部支部例会 (招待講演)

2015年

[原著論文]

Shogo Nakano, Seiji Okazaki, Erika Ishitsubo, Nobuhiro Kawahara, Hidenobu Komeda, Hiroaki Tokiwa and Yasuhisa Asano, Structural and computational analysis of peptide recognition mechanism of class-C type penicillin binding protein, alkaline D-peptidase from Bacillus cereus DF4-B, Scientific reports, 5, 13836 (doi: 10.1038/srep13836)

・Vu Thi Tuyet Lan, Keisuke Ito, Sohei Ito, Yasuaki Kawarasaki, Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV, Peptides, 54, 166-70

・Masumi Ohno, Keisuke Ito, Vu Thi Tuyet Lan, Masashi Kusubata, Chisa Tometsuka, Yoh-ichi Koyama, Takayasu Motoyama, Sohei Ito, Yasuaki Kawarasaki, "Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides", Peptides, 69, 115-117

・Aftab Ahmad, Yasuo Niwa, Shingo Goto, Kyoko Kobayashi, Masanori Shimizu, Sohei Ito, Yumiko Usui, Tsutomu Nakayama, Hirokazu Kobayashi, "Genome-Wide Screening of Salt Tolerant Genes by Activation-Tagging Using Dedifferentiated Calli of Arabidopsis and Its Application to Finding Gene for Myo-Inositol-1-P-Synthase" , PLoS ONE, doi: 10.1371/journal.pone.0115502

・Vu Thi Tuyet Lan, Keisuke Ito, Masumi Ohno, Takayasu Motoyama, Sohei Ito, Yasuaki Kawarasaki, "Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor", Food Chemistry, 175, 66-73

・Wisarut Payoungkiattikun, Seiji Okazaki, Shogo Nakano, Atsutoshi Ina, Aran H-Kittikun, Yasuhisa Asano, " In Silico Identification for α-Amino-ε-Caprolactam Racemases by Using I nformation on the Structure and Function Relationship", Applied Biochemistry and Biotechnology, in press, (doi: 10.1007/s12010-015-1647-6)

Shogo Nakano and Yasuhisa Asano, "Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software", Scientific reports, 5, 8193,    (doi: 10.1038/srep08193)

2014年

[原著論文]

・Yuko Terada, Takashi Hosono, Taiichiro Seki, Toyohiko Ariga, Sohei Ito, Masataka Narukawa, Tatsuo Watanabe, "Sulphur-containing compounds of durian activate the thermogenesis-inducing receptors TRPA1 and TRPV1", Food Chemistry, 157, 213-220

Shogo Nakano, Mohammad Dadashipour, and Yasuhisa Asano, "Structural and functional analysis of hydroxynitrile lyase from Baliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics", BBA Proteins and Proteomics, 1844, 2059-2067

・Kazuyuki Yasukawa, Shogo Nakano and Yasuhisa Asano, "Tailoring D-amino acid oxidase from the pig kidney to R-stereoselective amine oxidase and its use in the deracemization of α-methylbenzylamine", Angewandte Chemie International Edition, 53, 4428-4431

Shogo Nakano, Seiji Okazaki, Hiroaki Tokiwa and Yasuhisa Asano, "Binding of NAD+ and L-threonine induces stepwise structural and flexibility changes in Cupriavidus necator L-threonine dehydrogenase", Journal of Biological Chemistry, 289, 10445-10454

[日本語総説]

・浅野 泰久, 安川 和志, 中野 祥吾, "光学活性アミンを合成する酵素 -自然界にない新しいアミン酸化酵素の開発", 化学, 69, 45-49

[学会発表]

Shogo Nakano, Seiji Okazaki, Kazuyuki Yasukawa, Mohanmad Dadashipour, Hiroaki Tokiwa, Yasuhisa Asano, "Endeavors to bridge a gap between wet and dry science in enzyme engineering", Active Enzyme Molecule 2014 (oral presentation)

中野 祥吾, 岡崎 誠司, 浅野 泰久, "α/β hydrolase familyの一次配列情報を利用して構築した変異型S-ヒドロキシニトリルリアーゼの機能解析", 第87回日本生化学会大会 (3T09p-06, 口頭発表)

2013年

[原著論文]

・Seiji Okazaki, Shogo Nakano, Daisuke Matsui, Shusaku Akaji, Kenji Inagaki and Yasuhisa Asano, "X-Ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in l-lysine ε-oxidase from Marinomonas mediterranea", Journal of Biochemistry, 154, 233-236

[学会発表]

中野 祥吾, 岡崎 誠司, 常盤 広明, 浅野 泰久, "GalE-like L-スレオニン脱水素酵素に関するopen及びclosed型構造の存在と重要性について", 第86回日本生化学会大会 (2T18a-12, 口頭発表)

2012年

[原著論文]

Shogo Nakano, Misa Takahashi, Atsushi Sakamoto, Hiromichi Morikawa and Katsuo Katayanagi, "X-ray crystal structure of a mutant assimilatory nitrite reductase that shows sulfite reductase-like activity", Chemistry & Biodiversity, 9, 1989-1999

・Manami Hashimoto, Kazuya Momma, Satoshi Inaba, Shogo Nakano and Shin-ichi Aizawa, "The hydrophobic core of FliG domain II is the stabilizer in the Salmonella flagellar motor", Microbiology, 158, 2556-2567

Shogo Nakano, Misa Takahashi, Atsushi Sakamoto, Hiromichi Morikawa and Katsuo Katayanagi, "The reductive reaction mechanism of tobacco nitrite reductase derived from a combination of crystal structures and ultraviolet-visible microspectroscopy", Proteins: Structure, Function, and Bioinformatics, 80, 2035-2045

Shogo Nakano, Mamoru Sugihara, Risato Yamada, Katsuo Katayanagi, Shin-ichi Tate, "Structural implication for the impaired binding of W150A mutant LOX-1 to oxidized low density lipoprotein, OxLDL", BBA Proteins and Proteomics, 1824, 739-749

Shogo Nakano, Misa Takahashi, Atsushi Sakamoto, Hiromichi Morikawa and Katsuo Katayanagi, "Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on high-resolution structures", Protein Science, 21, 383-395


2011年以前

[原著論文]

・Keisuke Ito, Sohei Ito, Tatsuro Shimamura, Simone Weyand, Yasuaki Kawarasaki, Takumi Misaka, Keiko Abe, Takuya Kobayashi, Alexander D Cameron, So Iwata, Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans. J. Mol. Biol.29, 177-186 (2011)

・Yosuke Saito, Sohei Ito, Anna M Koltunow, Hiroshi Sakai, Crystallization and preliminary X-ray analysis of geraniol dehydrogenase from Backhousia citriodora (lemon myrtle). Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.67, 665-667 (2011)

Mizue Sugiura, Sohei Ito, Yosuke Saito, Yasuo Niwa, Anna M Koltunow, Osamu Sugimoto, Hiroshi Sakai, Molecular Cloning and Characterization of Linalool Synthase from Lemon Myrtle. Biosci. Biotechnol. Biochem., 75 (7), 1245-1248 (2011)

Takeshi Ishii, Tatsuya Ichikawa, Kanako Minoda, Koji Kusaka, Sohei Ito, Yukiko Suzuki, Mitsugu Akagawa, Kazuki Mochizuki, Toshinao Goda, Tsutomu Nakayama, Human serum albumin as an antioxidant in the oxidation of (-)-epigallocatechin gallate: participation of reversible covalent binding for interaction and stabilization. Biosci. Biotechnol. Biochem.75, 100-106 (2011)

Takahiro Shibata, Yuuki Kimura, Akihiro Mukai, Hitoshi Mori, Sohei Ito, Yukio Asaka, Sho Oe, Hiroshi Tanaka, Takashi Takahashi, Koji Uchida, Transthiocarbamolylation of Proteins by Thiolated Isohiocyanate. J. Biol. Chem.286, 42150-42161 (2011)

・伊藤圭祐、伊藤創平「Streptococcus mutans由来グルカンスクラーゼの立体構造解析 虫歯予防食品開発への分子基盤」化学と生物、 49巻10号、665-667

・伊藤圭祐、伊藤創平「抗う蝕物質の開発に向けたグルカンスクラーゼの構造解析」バイオサイエンスとインダストリー、69巻5号、387-388

・Satoru Yamaguchi, Giancarlo Aldini, Sohei Ito, Nozomi Morishaita, Takahiro Shibata, Giulio Cistoli, Marina Carini and Koji Uchida, Δ12-Prostaglandin J2 as a product and ligand of human serum albumin: Formation of an unusual covalent adduct at His146, Journal of American Chemical Society, 132; 824-832 (2010)

・Chika Wakita, Takuya Maeshima, Atsushi Yamazaki, Takahiro Shibata, Sohei Ito, Mitsugu Akagawa, Makoto Ojika, Junji Todoi and Koji Uchida, Stereochemical Configuration of 4-Hydroxy-2-nonenal-Cysteine Adducts and Their Stereoselective Formation in a Redox-regulated Protein, Journal of Biological Chemistry, 284, 28810-28822 (2009)

・Kenichiro Suzuki, Sohei Ito, Akiko Shimizu-Ibuka and Hiroshi Sakai, Crystal Structure of Pyruvate Kinase from Geobacillus stearothermophilus. Journal of Biochemistry144, 305-312 (2008)