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研究業績

2021

  • Hempstock W, Ishizuka N, Hayashi H. Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique. JoVE 2021
  • Hayashi H, Nagai H, Ohba KI, Soleimani M, Suzuki Y. Segmental differences in Slc26a3-dependent Cl- absorption and HCO3- secretion in the mouse large intestine in vitro in Ussing chambers. J Physiol Sci. 71(1), 5, 2021
  • Hirota C, Takashina Y, Ikumi N, Ishizuka N, Hayashi H, Tabuchi Y, Yoshino Y, Matsunaga T, Ikari A. Inverse regulation of claudin-2 and -7 expression by p53 and hepatocyte nuclear factor 4α in colonic MCE301 cells. Tissue Barriers. 9(1),1860409, 2021

2020

  • Hempstock W, Sugioka S, Ishizuka N, Sugawara T, Furuse M, Hayashi H. Angulin-2/ILDR1, a tricellular tight junction protein, does not affect water transport in the mouse large intestine. Sci rep. 10, 10374, 2020
  • Takashina Y, Ishizuka N, Ikumi N, Hayashi H, Manabe A, Hirota C, Tabuchi Y, Matsunaga T, Ikari A. Upregulation of Claudin-7 Expression by Angiotensin II in Colonic Epithelial Cells of Mice Fed With NaCl-Depleted Diets. Int J Mol Sci. 21(4), 1442, 2020
  • Nakayama M, Ishizuka N, Hempstock W, Ikari A, Hayashi H. Na+-Coupled Nutrient Cotransport Induced Luminal Negative Potential and Claudin-15 Play an Important Role in Paracellular Na+ Recycling in Mouse Small Intestine. Int J Mol Sci 21(2), 376, 2020

2019

  • Ishizuka N, Hayashi H. The Mode of Action of NHE3 Inhibitors in Intestinal Na+ Absorption. Gastroenterology Medicine & Research 4(1), 297-301, 2019

2018

  • Ishizuka N, Nakayama M, Watanabe M, Tajima H, Yamauchi Y, Ikari A, Hayashi H. Luminal Na+ homeostasis has an important role in intestinal peptide absorption in vivo. Am J Physiol. 315, 799-809, 2018

2017

  • Furukawa C, Ishizuka N, Hayashi H, Fujii N, Manabe A, Tabuchi Y, Matsunaga T, Endo S, Ikari A. Up-regulation of claudin-2 expression by aldosterone in colonic epithelial cells of mice fed with NaCl-depleted diets. Sci rep. 7(1), 12223, 2017

2015

  • Ikari A, Fujii N, Hahakabe S, Hayashi H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J. Hyperosmolarity-Induced Down-Regulation of Claudin-2 Mediated by Decrease in PKCβ-Dependent GATA-2 in MDCK Cells. J Cell Physiol. 230(11), 2776-87, 2015
  • Khan MR, Islam MT, Yazawa T, Hayashi H, Suzuki Y, Uwada J, Anisuzzaman AS, Taniguchi T. Muscarinic cholinoceptor-mediated activation of JNK negatively regulates intestinal secretion in mice. J Pharmacol Sci. 127(1), 150-3, 2015

2014

  • Ikari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J. Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells. J Biol Chem. 289(19), 13112-23, 2014
  • Ikehara O, Hayashi H, Waguri T, Kaji I, Karaki S, Kuwahara A, Suzuki Y. Luminal trypsin induces enteric nerve-mediated anion secretion in the mouse cecum. J Physiol Sci. 64(2), 119-28, 2014
  • Khan RI, Yazawa T, Anisuzzaman AS, Semba S, Ma Y, Uwada J, Hayashi H, Suzuki Y, Ikeuchi H, Uchino M, Maemoto A, Muramatsu I, Taniguchi T. Activation of focal adhesion kinase via M1 muscarinic acetylcholine receptor is required in restitution of intestinal barrier function after epithelial injury. Biochim Biophys Acta. 1842(4), 635-45, 2014

2013

  • Ikari A, Atomi K, Yamazaki Y, Sakai H, Hayashi H, Yamaguchi M, Sugatani J.Hyperosmolarity-induced up-regulation of claudin-4 mediated by NADPH oxidase-dependent H2O2 production and Sp1/c-Jun cooperation. Biochim Biophys Acta. 1833(12), 2617-27, 2013
  • Khan MRS , Anisuzzaman ASM , Semba S, Ma Y, Uwada J, Hayashi H, Suzuki Y, Takano T, Ikeuchi H, Uchino M, Maemoto A, Ushikubi F, Muramatsu I, Taniguchi T. M1 is a major subtype of muscarinic acetylcholine receptors on mouse colonic epithelial cells. J Gastroenterology 48 885-896 (2013)
  • Inoue H, Kobayashi-Yamakawa K, Suzuki Y, Nakano T, Hayashi H, Kuwano T. A case study on the association of variation of bitter-taste receptor gene TAS2R38 with the height, weight and energy intake in Japanese female college students. J Nutr Sci Vitaminol. 59 16-21 (2013)
  • Hayashi H, Tamura A, Krishnan D, Tsukita S, Suzuki Y, Kocinsky HS, Aronson PS, Orlowski J, Grinstein S, 9 and Alexander RT. Ezrin is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE3. PLoS One 8 (2), e55623 (2013)

2012

  • Ikehara O, Hayashi H, Waguri T, Kaji I, Karaki SI, Kuwahara A, Suzuki Y. Subepithelial trypsin induces enteric nerve-mediated anion secretion by activating proteinase-activated receptor 1 in the mouse cecum.　J Physiol Sci. 62.211-219, 2012
  • Ikari A, Atomi K, Takiguchi A, Yamazaki Y, Hayashi H, Hirakawa J, Sugatani J Enhancement of cell-cell contact by claudin-4 in renal epithelial Madin-Darby canine kidney cells. J Cell Biochem.113, 499-507, 2012
  • Hayashi H, Yamashita Y. Role of N-glycosylation in cell surface expression and protection against proteolysis of the intestinal anion exchanger SLC26A3. Am J Physiol Cell Physiol.302, C781-95, 2012

2011

  • Tamura A, Hayashi H, Imasato M, Yamazaki Y, Hagiwara A, Wada M, Noda T, Watanabe M, Suzuki Y, Tsukita S. Loss of Claudin-15, but Not Claudin-2, Causes Na+ Deficiency and Glucose Malabsorption in Mouse Small Intestine. Gastroenterology, 140, 913-923, 2011

2010

  • Koivusalo M, Welch C, Hayashi H, Scott CC, Kim M, Alexander T, Touret N, Hahn KM, Grinstein S. Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol. 188, 547-563, 2010
  • Ikehara O, Hayashi H, Watanabe Y, Yamamoto H, Mochizuki T, Hoshino M, Suzuki Y. Proteinase-activated receptors-1 and 2 induce electrogenic Cl- secretion in the mouse cecum by distinct mechanisms. Am J Physiol. 299, G115-125, 2010

2009

  • Hayashi H, Suruga K, Yamashita Y.Regulation of intestinal Cl-/HCO3- exchanger SLC26A3 by intracellular pH. Am J Physiol, 296, C1279-90, 2009
  • Inagaki-Tachibana E, Tsukahara T, Kaji K, Eguchi R, Kanazawa H, Hayashi H, Suzuki Y. Involvement of DNA fragmentation of enterocytes in mucosal injury to a mouse jejunum incubated in ussing chambers. Nagoya J Med Sci, 71, 11-18, 2009

2008

  • Inagaki-Tachibana E, Hayashi H, Suzuki Y.The electrophysiological properties of the mucosal barrier in the injured mouse jejunum in Ussing chambers. J Nutr Sci Vitaminol (Tokyo). Jun;54(3):269-71 2008
  • Hayashi H, Aharonovitz O, Alexander RT, Touret N, Furuya W, Orlowski J, Grinstein S. Na+/H+ exchange and pH regulation in the control of neutrophil chemokinesis and chemotaxis. Am J Physiol. 294, C526-34, 2008
  • Karaki S, Tazoe H, Hayashi H, Kashiwabara H, Tooyama K, Suzuki Y, Kuwahara A. Expression of the short-chain fatty acid receptor, GPR43, in the human colon. J Mol Histol, 39, 135-42, 2008
  • Inagaki-Tachibana E, Natori Y, Hayashi H, Suzuki Y. In vitro diffusion barriers of the mouse jejunum in ussing chambers. J Nutr Sci Vitaminol (Tokyo). 54, 30-8, 2008
  • Tamura A, Kitano Y, Hata M, Katsuno T, Moriwaki K, Sasaki H, Hayashi H, Suzuki Y, Noda T, Furuse M, Tsukita S, Tsukita S. Megaintestine in claudin-15-deficient mice. Gastroenterology, 134, 523-34, 2008

2007

  • Uchiyama H, Hayashi H, Tanji K, Sugimoto O, Suzuki Y. pH stat studies on bicarbonate secretion in the isolated mouse ileum. Biomed Res. 28, 239-46, 2007
  • Kawamata K, Hayashi H, Suzuki Y. Propionate absorption associated with bicarbonate secretion in vitro in the mouse cecum. Pflugers Arch, 454, 253-62, 2007

2006

  • Kawamata K, Hayashi H, Suzuki Y. Chloride-dependent bicarbonate secretion in the mouse large intestine. Biomed Res, 27, 15-21, 2006
  • Uchiyama H, Hayashi H, Suzuki Y. Functional characterization of Cl-/HCO3- exchange in villous cells of the mouse ileum. Biomed Res. 27, 265-74, 2006
  • Karaki S, Mitsui R, Hayashi H, Kato I, Sugiya H, Iwanaga T, Furness JB, Kuwahara A. Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine. Cell Tissue Res, 324, 353-60, 2006
  • Ikari A, Matsumoto S, Harada H, Takagi K, Hayashi H, Suzuki Y, Degawa M, Miwa M. Phosphorylation of paracellin-1 at Ser217 by protein kinase A is essential for localization in tight junctions. J Cell Sci, 119, 1781-9, 2006

2005

  • Inagaki E, Natori Y, Ohgishi Y, Hayashi H, Suzuki Y. Segmental difference of mucosal damage along the length of a mouse small intestine in an Ussing chamber. J Nutr Sci Vitaminol (Tokyo), 51, 406-12, 2005
  • Tamura A, Kikuchi S, Hata M, Katsuno T, Matsui T, Hayashi H, Suzuki Y, Noda T, Tsukita S, Tsukita S. Achlorhydria by ezrin knockdown: defects in the formation/expansion of apical canaliculi in gastric parietal cells. J Cell Biol, 169, 21-8, 2005

2004

  • Ikari A, Hirai N, Shiroma M, Harada H, Sakai H, Hayashi H, Suzuki Y, Degawa M, Takagi K. Association of paracellin-1 with ZO-1 augments the reabsorption of divalent cations in renal epithelial cells. J Biol Chem, 279, 54826-32, 2004
  • Hayashi H, Szaszi K, Coady-Osberg N, Furuya W, Bretscher AP, Orlowski J, Grinstein S. Inhibition and redistribution of NHE3, the apical Na+/H+ exchanger, by Clostridium difficile toxin B. J Gen Physiol, 123, 491-504, 2004

2003

  • Hayashi H, Suzuki T, Yamamoto T, Suzuki Y. Cholinergic inhibition of electrogenic sodium absorption in the guinea pig distal colon Am J Physiol, 284, G617-628, 2003

2002

  • Hayashi H, Szaszi K, Coady-Osberg N, Orlowski J, Kinsella JL, Grinstein S. A slow pH-dependent conformational transition underlies a novel mode of activation of the epithelial Na+/H+ exchanger-3 isoform. J Biol Chem, 277, 11090-11096, 2002
  • Wang C, Hayashi H, Harrison R, Chiu B, Chan JR, Ostergaard HL, Inman RD, Jongstra J, Cybulsky MI, Jongstra-Bilen J. Modulation of Mac-1 (CD11b/CD18)-mediated adhesion by the leukocyte-specific protein 1 is key to its role in neutrophil polarization and chemotaxis. J Immunol, 169, 415-23, 2002
  • Hayashi H, Szaszi K, Grinstein S. Multiple modes of regulation of Na+/H+ exchangers.Ann N Y Acad Sci, 976:248-58, 2002

2001

  • Tsuchiya Y, Hayashi H, Suzuki Y. Na+-dependent recovery of intracellular pH from acid loading in mouse colonic crypt cells. Tohoku J Exp Med, 193, 1-11, 2001

1999

  • Sato Y, Hanai H, Nogaki A, Hirasawa K, Kaneko E, Hayashi H, Suzuki Y. Role of the vasopressin V-1 receptor in regulating the epithelial functions of the guinea pig distal colon. Am J Physiol, 277, G819-828, 1999
  • Koyama K, Sasaki I, Naito H, Funayama Y, Fukushima K, Unno M, Matsuno S, Hayashi H, Suzuki Y. Induction of epithelial Na+ channel in rat ileum after proctocolectomy. Am J Physiol, 276, G975-984, 1999

1998

  • Hayashi H, Suzuki Y. Regulation of intracellular pH during H+-coupled oligopeptide absorption in enterocytes from guinea-pig ileum. J Physiol. 511, 573-586, 1998

1996

  • Kitaoka S, Hayashi H, Yokogoshi H, Suzuki Y. Transmural potential changes associated with the in vitro absorption of theanine in the guinea pig intestine. Biosci Biotechnol Biochem, 60, 1768-1771, 1996
  • Ikuma M, Hanai H, Kaneko E, Hayashi H, Hoshi T. Effects of aging on the regulation of intracellular pH in the rat jejunum. J Gerontol A Biol Sci Med Sci, 51, B346-353, 1996
  • Ikuma M, Hanai H, Kaneko E, Hayashi H, Hoshi T. Effects of aging on the microclimate pH of the rat jejunum. Biochim Biophys Acta, 1280, 19-26, 1996

1994

  • Dougen M, Hayashi H, Yajima T, Suzuki Y : Stimulation of bicarbonate secretion by luminal short-chain fatty acid in the rat and human colon in vitro, Jpn J Physiol, 44, 519-531, 1994

1992

  • Hayashi H, Hoshi T. Properties of active magnesium flux across the small intestine of the guinea pig. Jpn J Physiol, 42, 561-75, 1992