研究成果は英語原著論文として世界に向けて発信していきます。他の研究者の厳しい査読を経るため論文掲載までには大変な苦労がありますが、一度発表した論文は、時代を超えて世界中の人に見てもらえる可能性があります。もちろん研究が進めば学部生、大学院生にも論文執筆の機会があります。

M. Masuda, Y. Terada, R. Tsuji, S. Nakano, K. Ito†. Time-series sensory analysis provided important TI parameters for masking the beany flavor of soymilk. Foods, 2023, 12, 2752.

F. Nakagawa, M. Kikkawa, S. Chen, Y. Miyashita, N. Hamaguchi-Suzuki, M. Shibuya, S. Yamashita, L. Nagase, S. Yasuda, M. Shiroishi, T. Senda, K. Ito, T. Murata, S. Ogasawara. Anti-nanodisc antibodies specifically capture nanodiscs and facilitate molecular interaction kinetics studies for membrane protein. Sci. Rep., 2023, 13, 11627.

Y. Terada, K. Tanaka, M. Matsuyama, M. Fujitani, M. Shibuya, Y. Yamamoto, R. Kato, K. Ito. Collection of data variation using a high-throughput image-based assay platform facilitates data-driven understanding of TRPA1 agonist diversity. Applied Sci. 2022 12 1622.

T. Ikeya, Y. Terada, Y. Morimitsu, K. Kubota, K. Ito, T. Watanabe. 1'-Acetoxychavicol acetate, a potent transient receptor potential ankyrin 1 agonist derived from Thai ginger, prevents visceral fat accumulation in mice fed with a high-fat and high-sucrose diet. Biosci. Biotechnol. Biochem. 2021 85 2191-2194.

I. Ojiro, H. Nishio, T. Yamazaki-Ito, S. Nakanoa, S. Ito, Y. Toyohara, T. Hiramoto, Y. Terada, K. Ito. Trp-Trp acts as a multi-functional blocker for human bitter taste receptors, hTAS2R14, hTAS2R16, hTAS2R43, and hTAS2R46. Biosci. Biotechnol. Biochem. 2021 85 1526-1529.

S. Tsuchiya, Y. Terada, M. Matsuyama, T. Yamazaki-Ito, K. Ito. A new screening method for identifying chemosensory receptors responding to agonist. Biosci. Biotechnol. Biochem. 2021 85 1521-1525.

M. Matsuyama, Y. Terada, T. Yamazaki-Ito, K. Ito. A luminescence-based human TRPV1 assay system for quantifying pungency in spicy foods. Foods 2021 10 151.

K. Ito, M. Koike, Y. Kuroda, T. Yamazaki-Ito, Y. Terada, T. Ishii, Y. Nakamura, T. Watanabe, Y. Kawarasaki. Bitterness-masking peptides for epigallocatechin gallate identified through peptide array analysis. Food Sci. Technol. Res. 2021 27 221-228.

M. Tanaka, K. Ito, T. Matsuura, Y. Kawarasaki, K. Gomi. Identification and distinct regulation of three di/tripeptide transporters in Aspergillus oryzae. Biosci. Biotechnol. Biochem. 2021 85 452-463.

K. Ito, T. Hosoya, T. Yamazaki-Ito, Y. Terada, Y. Kawarasaki. Dipeptidyl peptidase IV inhibitory dipeptides contained in hydrolysates of green tea grounds. Food Sci. Technol. Res. 2021 27 329-334.

M. Arakia, N. Kanegawa, H. Iwata, Y. Sagae, K. Ito, K. Masuda, Y. Okunoa. Hydrophobic interactions at subsite S1' of human dipeptidyl peptidase IV contribute significantly to the inhibitory effect of tripeptides. Heliyon 2020 6 e04227.

M. Yamamoto, Y. Terada, T. Motoyama, M. Shibata, T. Saito, K. Ito. N-terminal [Glu]3 moiety of γ-glutamyl peptides contributes largely to the activation of human calcium-sensing receptor, a kokumi receptor. Biosci. Biotechnol. Biochem. 2020 84 1497-1500.

M. Yamamoto, Y. Terada, T. Motoyama, T. Saito, K. Ito. Sweet proteins lysozyme and thaumatin are protein-type agonists for the calcium-sensing receptor. Biochem. Biophys. Res. Commun. 2019 521 227-231.

Y. Terada, R. Yamashita, N. Ihara, T. Yamazaki-Ito, Y. Takahashi, H. Masuda, S. Sakuragawa, S. Ito, K. Ito, T. Watanabe. Human TRPA1 activation by terpenes derived from the essential oil of daidai, Citrus aurantium L. var. daidai Makino. Biosci. Biotechnol. Biochem. 2019 83 1721-1728.

H. Su, K. Ito, Y. Kawarasaki, H. Morita, H. Nose, K. Ikeda, F. Nakadouzono, M. Gokuden, S. Kamiyama, A. Tokaji, Y. Rikitake, T. Kawaguchi, K. Umekita, S. Oishi, F. Abe, T. Kanda, H. Kawabata, S. Ando, N. Ohashi. Insight of diagnostic performance using B-cell epitope antigens derived from triple P44-related proteins of Anaplasma phagocytophilum. Diagn. Microbiol. Infect. Dis. 2019 95 125-130. 

T. Hashidume, T. Sakano, A. Mochizuki, K. Ito, S. Ito, Y. Kawarasaki, N. Miyoshi. Identification of soybean peptide leginsulin variants in different cultivars and their insulin-like activities. Sci. Rep. 2018 8 16847.

M. Tanaka, Y. Matsuo, H. Yamakage, S. Masuda, Y. Terada, K. Muranaka, H. Wada, K. Hasegawa, A. Shimatsu, N. Satoh-Asahara. Differential effects of GLP-1 receptor agonist on foam cell formation in monocytes between non-obese and obese subjects. Metabolism 2016 65 1-11.

H. Mizuno, Y. Miyazaki, K. Ito, K. Todoroki, J.Z. Min, T. Toyo'oka. A rapid and sensitive detection of D-Aspartic acid in Crystallin by chiral derivatized liquid chromatography mass spectrometry. J. Chromatogr. A 2016 1467 318-325.

Y. Kuroda, R. Ikeda, T. Yamazaki, K. Ito, K. Uda, K. Wakabayashi, T. Watanabe. Activation of human bitter taste receptors by polymethoxylated flavonoids. Biosci. Biotechnol. Biochem. 2016 4 1-4.

T. Watanabe, Y. Terada. Food compounds activating thermosensitive TRP channels in asian herbal and medicinal foods. J. Nutr. Sci. Vitaminol. (Tokyo) 2015 61 S86-88.

Y. Terada, H. Masuda, T. Watanabe. Structure-activity relationship study on isothiocyanates: comparison of TRPA1-activating ability between allyl isothiocyanate and specific flavor components of wasabi, horseradish, and white mustard. J. Nat. Prod. 2015 78 1937-1941.

M. Ohno, K. Ito, V.T.T. Lan, M. Kusubata, C. Tometsuka, Y. Koyama, T. Motoyama, S. Ito, Y. Kawarasaki. Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides. Peptides 2015 69 115-117.

V.T.T. Lan, K. Ito, M. Ohno, T. Motoyama, S. Ito, Y. Kawarasaki. Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor. Food Chem. 2015 15(175) 66-73.

Y. Terada, M. Kitajima, F. Taguchi, H. Takayama, S. Horie, T. Watanabe. Identification of indole alkaloid structural units important for stimulus-selective TRPM8 inhibition: SAR study of naturally occurring iboga derivatives. J. Nat. Prod. 2014 77 1831-1838.

Y. Terada, S. Horie, H. Takayama, K. Uchida, M. Tominaga, T. Watanabe. Activation and inhibition of thermosensitive TRP channels by voacangine, an alkaloid present in Voacanga africana, an African tree. J. Nat. Prod. 2014 77 285-297.

Y. Terada, T. Hosono, T. Seki, T. Ariga, S. Ito, M. Narukawa, T. Watanabe. Sulphur-containing compounds of durian activate the thermogenesis-inducing receptors TRPA1 and TRPV1. Food Chem. 2014 157 213-220.

T. Yamazaki, M. Sagisaka, R. Ikeda, T. Nakamura, N. Matsuda, T. Ishii, T. Nakayama, T. Watanabe. The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins. Biosci. Biotechnol. Biochem. 2014 78 1753-1756.

V.T.T. Lan, K. Ito, S. Ito, Y. Kawarasaki. Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV. Peptides 2014 54 166-170.

T. Kurose, Y. Saito, K. Kimata, Y. Nakagawa, A. Yano, K. Ito, Y. Kawarasaki. Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: a sub-milligram production of "difficult-to-express" secretory protein. J. Biosci. Bioeng. 2014 117(6) 659-663.

K. Ito, A. Hikida, V.T.T. Lan, T. Motoyama, S. Kitagawa, Y. Yoshikawa, R. Kato, Y. Kawarasaki. Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library. Nature Commun. 2013 4(2502).

M. Hoshi, Y. Ohki, K. Ito, T. Tomita, T. Iwatsubo, Y. Ishimaru, K. Abe, T. Asakura. Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana. BMC Biochemistry 2013 14(16).

A. Hikida, K. Ito, T. Motoyama, R. Kato, Y. Kawarasaki. Systematic analysis of a dipeptide library for inhibitor development using human dipeptidyl peptidase IV produced by a Saccharomyces cerevisiae expression system. Biochem. Biophys. Res. Commun. 2013 430(4) 1217-1222.

T. Yamazaki, M. Narukawa, M. Mochizuki, T. Misaka, T. Watanabe. Activation of the hTAS2R14 human bitter-taste receptor by (-)-epigallocatechin gallate and (-)-epicatechin gallate. Biosci. Biotechnol. Biochem. 2013 77 1981-1983.

K. Ito, A. Hikida, S. Kitagawa, T. Misaka, K. Abe, Y. Kawarasaki. Soy peptides enhance heterologous membrane protein expression during the exponential growth phase of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 2012 76(3) 628-631.

K. Kimata, M. Yamaguchi, Y. Saito, H. Hata, K. Miyake, T. Yamane, Y. Nakagawa, A. Yano, K. Ito, Y. Kawarasaki. High cell-density expression system: A novel method for extracellular production of "difficult-to-express" proteins. J. Biosci. Bioeng. 2012 113(2) 154-159.

Y. Terada, M. Narukawa, T. Watanabe. Specific hydroxy fatty acids in royal jelly activate TRPA1. J. Agric. Food Chem. 2011 59 2627-2635.

A. Koizumi, A. Tsuchiya, K. Nakajima, K. Ito, A. Shimizu-Ibuka, T. Asakura, L. Briand, T. Misaka, K. Abe. Human sweet taste receptor mediates acid-induced sweetness of miraculin. Proc. Natl. Acad. Sci. USA 2011 108(40) 16819-16824.

K. Nakajima, A. Koizumi, K. Iizuka, K. Ito, Y. Morita, T. Koizumi, T. Asakura, A. Shimizu-Ibuka, T. Misaka, K. Abe. Non-acidic compounds induce the intense sweet taste of neoculin, a taste-modifying protein. Biosci. Biotechnol. Biochem. 2011 75(8) 1600-1602.

H. Asada, T. Uemura, T. Yurugi-Kobayashi, M. Shiroishi, T. Shimamura, H. Tsujimoto, K. Ito, T. Sugawara, T. Nakane, N. Nomura, T. Murata, T. Haga, S. Iwata, T. Kobayashi. Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis. Microb. Cell Fact. 2011 22;10:24.

K. Nakajima, K. Yokoyama, T. Koizumi, A. Koizumi, T. Asakura, T. Terada, K. Masuda, K. Ito, A. Shimizu-Ibuka, T. Misaka, K. Abe. Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein. PLoS ONE 2011 6(4) e19448.

N. Tokuda, K. Igarashi, T. Shimamura, T. Yurugi-Kobayashi, M. Shiroishi, K. Ito, T. Sugawara, H. Asada, T. Murata, N. Nomura, S. Iwata, T. Kobayashi. Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium. Protein Expr. Purif. 2011 79(1) 81-87.

T. Asakura, M. Miyano, H. Yamashita, T. Sakurai, K. Nakajima, K. Ito, T. Misaka, Y. Ishimaru, K. Abe. Analysis of taste continuity - case of sweet proteins. Flav. Fragr. J. 2011 26(4) 274-278.

K. Ito, S. Ito, T. Shimamura, S. Weyand, Y. Kawarasaki, T. Misaka, K. Abe, T. Kobayashi, A.D. Cameron, S. Iwata. Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans. J. Mol. Biol. 2011 408(2) 177-186.

K. Ito, T. Sugawara, A. Koizumi, K. Nakajima, A. Shimizu-Ibuka, M. Shiroishi, H. Asada, T. Yurugi-Kobayashi, T. Shimamura, T. Asakura, K. Masuda, M. Ishiguro, T. Misaka, S. Iwata, T. Kobayashi, K. Abe. Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin. Biochim. Biophys. Acta - General Subjects 2010 1800(9) 986-992.

T. Sakurai, T. Misaka, M. Ishiguro, K. Masuda, T. Sugawara, K. Ito, T. Kobayashi, S. Matsuo, Y. Ishimaru, T. Asakura, K. Abe. Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16. J. Biol. Chem. 2010 285(36) 28373-28378.

K. Ito, S. Ito, T. Shimamura, Y. Kawarasaki, K. Abe, T. Misaka, T. Kobayashi, S. Iwata. Crystallization and preliminary X-ray analysis of a Glucansucrase from the dental caries pathogen Streptococcus mutans. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2010 66(Pt 9) 1086-1088.

K. Ito, T. Sugawara, A. Koizumi, K. Nakajima, A. Shimizu-Ibuka, M. Shiroishi, H. Asada, T. Yurugi-Kobayashi, T. Shimamura, T. Asakura, T. Misaka, S. Iwata, T. Kobayashi, K. Abe. Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein. Biotechnol. Lett. 2010 33(1) 103-107.

M. Miyano, H. Yamashita, T. Sakurai, K. Nakajima, K. Ito, T. Misaka, Y. Ishimaru, K. Abe, T. Asakura. Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model. J. Agric. Food Chem. 2010 58(22) 11870-11875.